Fig. 5: Effect of Notch blockade on bacterial colonization, neutrophil recruitment, and myeloperoxidase (MPO) activity.
From: DCLK1 isoforms and aberrant Notch signaling in the regulation of human and murine colitis
Ai Electron microscopy of distal colons isolated from uninfected (N), CR-infected (CR), CR + DBZ-treated (CR + DBZ), or CR + DBZ plus antibiotics (CR + DBZ + Abx)-treated Rag-1−/− mice. Loss of microvilli structure in response to CR infection was exacerbated following Notch inhibition in the CR + DBZ group with clear bacterial invasion into the sub-mucosa. Antibiotics (Abx) treatment restored the microvilli. Aii represents the EM of distal colon from the CR + DBZ group showing interaction of bacteria with immune cells (6 mice/group; n = 3 independent experiments). B Representative images showing bacterial staining by anti-CR antibody (αCR). C Flow cytometry for Gr1(Ly6G)+ cells was performed in the colon tissues of uninfected (N), CR-infected (CR), or CR + DBZ-treated (CR + DBZ) Rag-1−/− mice. D MPO activity was measured in the colonic homogenates of the indicated groups using a Fluoro MPO Detection Kit (6 mice/group; n = 3 independent experiments).
