Fig. 6: Microbial dysbiosis coincides with downregulation of crypt DCLK1 following Notch blockade.
From: DCLK1 isoforms and aberrant Notch signaling in the regulation of human and murine colitis
A Fecal samples from uninfected (N), CR-infected (CR), and CR + DBZ-treated mice were subjected to 16S rDNA sequencing and relative abundance of phyla and families were compared. Each chart represents the taxonomic composition in the indicated groups (n = 10 mice/group). B Principal coordinate analysis (PCoA) based on weighted UniFrac distances between bacterial communities. Please note significant separation of microbial communities in fecal samples from CR + DBZ mice when compared to either uninfected (N) or CR-infected (CR) mice (P < 0.05). C The bacteria in the colonic tissues of N, CR, CR + DBZ, and CR + DBZ + Abx-treated mice were detected by FISH using a general bacterial 16S probe (red, TexasRed-Eub338; bar = 100 μm; n = 3 independent experiments). DAPI was used as counterstain. D Representative images of DCLK1 staining in the distal colons of the indicated groups (n = 10 mice/group). E Flow cytometry showing percentages of DCLK1+ cells isolated from the groups of mice indicated (n = 10 mice/group).
