Fig. 2: Identification of endothelial cell clusters under disturbed blood flow.
From: Single-cell RNA-seq reveals cellular heterogeneity of mouse carotid artery under disturbed flow
A t-distributed stochastic neighbor embedding (t-SNE) plot of EC subpopulations from the left carotid artery of wild-type mice 7 days after partial carotid ligation (PCL) or without PCL (control). B t-SNE plot of five distinct EC clusters from the PCL and control. C The violin plots of Dkk2 (an EC 4-associated marker) expression in all five EC clusters. D The violin plots of Cd36 (an EC 5-associated marker) expression in all five EC clusters. E Representative en face immunostaining images for CD36 and DKK2 in the left carotid artery with partial carotid ligation (right) or without PCL (control) (left) (red: CD31; green: DKK2; blue: CD36). bar = 20 µm. The magnified image was shown on the right, where the arrows indicated the co-localization of the related proteins. bar = 10 µm. F The heatmap showing the differences in biological processes by GSVA enrichment scores among the different EC clusters. G GSVA enrichment scores by using upregulated gene set (Fosl2, Ctgf, Ctps, Lmo4, Angpt2, Icam1, Bmp4, and Sema7a) under d-flow. H Branched pseudotime trajectory, each cell being colored by its pseudotime value (left) and its seurat clusters (right). I The heatmap showing variable genes along the pseudotime trajectory. The X-axis represents cells ordered by the pseudotime (from left to right) and different colors correspond to the scaled expression of each gene in each cell (from Monocle).
