Fig. 4: Distinct macrophage populations and their expression signatures.
From: Single-cell RNA-seq reveals cellular heterogeneity of mouse carotid artery under disturbed flow
A t-distributed stochastic neighbor embedding (t-SNE) plot of macrophages (Mφ)-dendritic cells (DCs) populations from the left carotid artery of wild-type mice 7 days after partial carotid ligation (PCL) or without PCL (control). B t-SNE plot of four Mφ-DCs clusters from the PCL and control. C The heatmap showing 10 most upregulated genes in each defined cluster. D Representative immunofluorescence microscopy images for BIRC5 and TREM2 in the left carotid artery with partial carotid ligation. The left panel is the control group, bar = 50 µm. The middle panel is the merged images either stained with CD68 (green) + BIRC5 (red) (top) or CD68 (green) + TREM2 (red) (bottom). The right panel is the enlarged image of the box in the middle panel. The arrow represents the co-localization of the related proteins. bar = 10 µm. E The heatmap of GSVA enrichment scores in biological processes among four distinct populations. F Cell-cycle analysis. Mφ/DCs were evaluated by calculating cell-cycle phase scores based on the canonical markers. G The violin plot of Ki67 expression in all four clusters. H Branched pseudotime trajectory, each cell population being colored by its pseudotime value (left) and its seurat clusters (right).
