Fig. 5: Overexpression of GSTA2 promoted HCC growth and invasion.

A The expression level of GSTA2 mRNA and protein in GSTA2-overexpressing HCC cells. B MTT assay of HCC cells after endogenously overexpressing GSTA2 by lentivirus (Lent-GSTA2) or administrating human recombinant GSTA2 protein (rGSTA2). C Colony-forming assay. D Matrigel invasion assay. Scale bar: 200 μM. E Wound healing migration assay. Scale bar: 1000 μM. F Western blot analysis of EMT-associated proteins.. Control, Hep3B-control cells; Lenti-GSTA2, Hep3B-LentiGSTA2 cells; rGSTA2, human recombinant GSTA2 protein. *P < 0.05; **P < 0.01.