Fig. 1: Sensitivity to ferroptosis was varied among liver cancer cells.
From: TRIB2 desensitizes ferroptosis via βTrCP-mediated TFRC ubiquitiantion in liver cancer cells
A Percentage of death cells in liver cancer cells, as indicated following treating with RSL3 (5 µM, 12 h) or erastin (10 µM, 12 h) with or without DFO (25 µM, 12 h), as measured by staining with Sytox green following flow cytometry. B PI staining of liver cancer cells as indicated following treating with RSL3 (5 µM, 6 h) or erastin (10 µM, 6 h) with or without DFO (25 µM, 6 h). Scale bar, 50 μM. C Cell morphology changes of Bel-7404 and SK-Hep1 cells following treating with RSL3 (5 µM) or erastin (10 µM) with or without DFO (25 µM) for indicated hours. Scale bar, 50 μM. D Cell death, lipid ROS generation and cell viability in Bel-7404 and SK-Hep1 cells following treating with the indicated concentration of RSL3 or erastin for 12 h, as measured by Sytox green and C11-BODIPY staining following flow cytometry and a ATP detection kit. Data were analyzed by one-way ANOVA and expressed as mean ± SD from three independent experiments. NS non-significance; **P < 0.01; ***P < 0.001; ****P < 0.0001.
