Fig. 5: LINC00467 promotes TGCT cell migration and invasion. | Cell Death Discovery

Fig. 5: LINC00467 promotes TGCT cell migration and invasion.

From: Integrated analysis of high-throughput sequencing data reveals the key role of LINC00467 in the invasion and metastasis of testicular germ cell tumors

Fig. 5

A qRT-PCR assay was used to detect LINC00467 overexpression in the cell lines. B qRT-PCR assay was used to detect the silencing efficiency of LINC00467 siRNA in the cell lines. The relative expression of LINC00467 in each group was calculated using β-actin as an internal reference and normalized to 1 in the NC siRNA group. C, E Transwell cell migration assays were used to determine the effects of LINC00467 overexpression (C) and the silencing of LNC00467 (E) on the migration ability of TGCT cells. D, F Transwell cell invasion assays were used to determine the effects of LINC00467 overexpression (D) and the silencing of LNC00467 (F) on the migration ability of TGCT cells. G, H Clone formation assay to detect the effect of LINC00467 overexpression (G) and the silencing of LNC00467 (H) on the colony-formation ability of TGCT cells. *P < 0.05; **P < 0.01; ***P < 0.001; n.s. no significance. All experiments were performed in triplicate. Scale bar = 100 μm.

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