Fig. 6: LINC00467 regulates AKT3-dependent AKT phosphorylation to promote the migration and invasion abilities of TGCT cells. | Cell Death Discovery

Fig. 6: LINC00467 regulates AKT3-dependent AKT phosphorylation to promote the migration and invasion abilities of TGCT cells.

From: Integrated analysis of high-throughput sequencing data reveals the key role of LINC00467 in the invasion and metastasis of testicular germ cell tumors

Fig. 6

A KEGG enrichment analysis of the genes co-expressed with LNC00467 in the Meturquoise module is shown in Fig. 4B. B, C Western blotting analysis of the change in the p-AKT level after the overexpression of LNC00467 (B) and LINC00467 silencing (C). D Data from the GEPIA database were used to determine the correlation between LINC00467 expression and AKT3 expression. E qRT-PCR and western blotting were used to determine the mRNA and protein levels of AKT3 after LINC00467 silencing. F Transwell cell migration and invasion assays were used to determine the effect of silencing on the migration and invasion abilities of TGCT cells. G Transwell cell migration and invasion assays were used to detect the effect of LINC00467 overexpression and simultaneous silencing of AKT3 on the migration and invasion abilities of TGCT cells. **P < 0.01; ***P < 0.001. All assays were performed in triplicate. Scale bar = 100 μm.

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