Fig. 1: HDAC4 is up-regulated in rat myocardial tissues after MIRI and its silencing alleviates myocardial injury in vivo. | Cell Death Discovery

Fig. 1: HDAC4 is up-regulated in rat myocardial tissues after MIRI and its silencing alleviates myocardial injury in vivo.

From: Histone deacetylase HDAC4 participates in the pathological process of myocardial ischemia-reperfusion injury via MEKK1/JNK pathway by binding to miR-206

Fig. 1

A Analysis of the myocardial injury area of rats by TTC staining (n = 5 for rat in each group). B Representative images of the myocardial injuries evaluated by H&E staining (n = 10; scale bar = 50 μm). C Apoptosis of cardiomyocytes detected by TUNEL assay (n = 10; scale bar = 50 μm). D Serum levels of IL-6 and TNF-α in peripheral blood of rats measured by ELISA (n = 10). E Determination of SOD, GSH, and MDA contents in rat myocardial tissues (n = 10). F Protein expression of HDAC4 in rat myocardial tissues detected by western blot analysis, normalized to GAPDH (n = 10). G Protein expression of Bax, Bcl-2, and cleaved Caspase-3 in rat myocardial tissues detected by western blot analysis, normalized to GAPDH (n = 10). *p < 0.05 vs. the sham-operated rats; #p < 0.05 vs. the rats with MIRI. Data were all measurement data and expressed as mean ± standard derivation. Comparisons among multiple groups were analyzed by one-way ANOVA, followed by Tukey’s post hoc test.

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