Fig. 2: Silencing of HDAC4 protects cardiomyocytes from IRI in vitro. | Cell Death Discovery

Fig. 2: Silencing of HDAC4 protects cardiomyocytes from IRI in vitro.

From: Histone deacetylase HDAC4 participates in the pathological process of myocardial ischemia-reperfusion injury via MEKK1/JNK pathway by binding to miR-206

Fig. 2

A HDAC4 silencing efficiency and the expression of HDAC5, HDAC7, and HDAC9 in cardiomyocytes evaluated by western blot analysis, normalized to GAPDH. B The levels of inflammatory factors (IL-6 and TNF-α) in cardiomyocytes determined by ELISA. C The levels of SOD, GSH, and MDA in the cardiomyocytes. D Apoptosis of cardiomyocytes detected by flow cytometry. E The expression of Bax, Bcl-2, and cleaved Caspase-3 protein in cardiomyocytes determined by western blot analysis, normalized to GAPDH. *p < 0.05 vs. the cardiomyocytes transfected with sh-NC. Data were all measurement data and expressed as mean ± standard derivation. Comparisons between two groups were analyzed by unpaired t-test. The experiment was conducted three times independently.

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