Fig. 4: Silencing of HDAC4 elevates the expression of miR-206 and further inhibits the activation of the MEKK1/JNK pathway in cardiomyocytes from IRI rats.
A The binding of HDAC4 to the promoter region of miR-206 identified by ChIP assay. B Expression of miR-206 in sh-HDAC4-treated cardiomyocytes determined by RT-qPCR. C Protein expression of MEKK1, JNK and p-JNK in sh-HDAC4-treated cardiomyocytes measured by western blot analysis, normalized to GAPDH. D The interaction between miR-206 and MEKK1 detected by dual luciferase reporter gene assay. E Protein expression of MEKK1 and JNK in miR-206 mimic-treated cardiomyocytes determined by western blot analysis, normalized to GAPDH. F Expression of miR-206 in cardiomyocytes transfected with sh-HDAC4 or sh-HDAC4 + miR-206 inhibitor measured by RT-qPCR. G The expression of MEKK1 and p-JNK in the cardiomyocytes transfected with sh-HDAC4 or sh-HDAC4 + miR-206 inhibitor measured by western blot analysis, normalized to GAPDH. *p < 0.05 vs. the cardiomyocytes transfected with sh-NC, NC mimic, NC inhibitor, or sh-HDAC4 + NC inhibitor. Data were measurement data and expressed as mean ± standard derivation. Comparisons between two groups were analyzed by the unpaired t-test, and comparisons among multiple groups were analyzed by one-way ANOVA, followed by Tukey’s host hoc test. The experiment was conducted three times independently.
