Fig. 5: BCKDK inactivation suppresses mitochondrial function and complex protein expression. | Cell Death Discovery

Fig. 5: BCKDK inactivation suppresses mitochondrial function and complex protein expression.

From: Inhibiting BCKDK in triple negative breast cancer suppresses protein translation, impairs mitochondrial function, and potentiates doxorubicin cytotoxicity

Fig. 5

A Heatmap for genes involved in mitochondrial biogenesis, structure, and function significantly differentially regulated by BCKDK knockdown. B Immunoblot and densitometric quantification of complex I–V proteins in MDA-MB231 whole cell lysates transfected with siCON, siBDK#1, or siBDK#2 for 72 h. Data presented as mean ± S.D. Statistical analysis was performed using a two-way ANOVA followed by a Tukey’s multiple comparison test; *p < 0.05, **p < 0.01, ****p < 0.0001 as indicated. C–H Mitochondrial function measured in BCKDK depleted MDA-MB231 cells using extracellular flux analyzer in the presence of 25 mM glucose. C Basal OCR, D ATP production measured in MDA-MB231 cells transfected with siCON or siBDK#2. E Basal and maximal OCR, F ATP production, G spare capacity, and H proton leak measured in MDA-MB231 cells treated with 250 or 500 µM BT2 for 20 h. B, *siCON vs siBDK#1, #siCON vs siBDK#2; C–D, *within groups (DMSO vs DOX), #between groups (siCON vs siBDK#2); E–H, *VEH vs 250μM BT2, #VEH vs 500μM BT2. Data presented as mean ± S.D. Statistical analysis was performed using a was performed using Student’s t test; *p < 0.05, **p < 0.01, ****p < 0.0001 as indicated.

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