Fig. 4: circPDCD11 was identified to target miR-432-5p. | Cell Death Discovery

Fig. 4: circPDCD11 was identified to target miR-432-5p.

From: CircRNA circ-PDCD11 promotes triple-negative breast cancer progression via enhancing aerobic glycolysis

Fig. 4

A The nuclear and cytoplasmic fractions of circPDCD11 were assessed using RT-qPCR in TNBC cells (MDA-MB-468, BT549). B The downstream target element of circPDCD11 was predicted using CircInteractome (https://circinteractome.nia.nih.gov/), which revealed that miR-432-5p displayed the complementary binding sites for circPDCD11. C A luciferase reporter assay demonstrated the intermolecular interaction (firefly/Renilla) in 293 T cells transfected with circPDCD11 wild type or mutant and miR-432-5p mimics or controls. D RT-PCR demonstrated the expression of miR-432-5p in MDA-MB-468 cells transfected with a circPDCD11 knockdown (sh-circPDCD11) and in BT549 cells overexpressing circPDCD11 (circPDCD11 up). E Clinical correlation analysis calculated by Pearson’s correlation reflected the correlation between circPDCD11 and miR-432-5p in clinical samples (32 samples). The data are presented as the means ± SD. Asterisks indicate significant differences (**p < 0.01).

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