Fig. 5: LDHA was identified to be targeted by circPDCD11/miR-432-5p. | Cell Death Discovery

Fig. 5: LDHA was identified to be targeted by circPDCD11/miR-432-5p.

From: CircRNA circ-PDCD11 promotes triple-negative breast cancer progression via enhancing aerobic glycolysis

Fig. 5

A Online bioinformatics predictive tools identified the potential targets of circPDCD11/miR-432-5p. B The multiple complementary binding sites within miR-432-5p and LDHA, including wild type and mutant. C The luciferase reporter assay demonstrated the close interaction between wild-type LDHA mRNA 3′-UTR and miR-432-5p. The fluorescence intensity was assessed. D Western blot analysis assessed the LDHA protein level in BT549 cells transfected with circPDCD11 overexpression and/or cotransfected with miR-432-5p mimics. E Clinical correlation analysis calculated by Pearson’s correlation showed the correlation between circPDCD11 and LDHA. The data are presented as the means ± SD. Asterisks indicate significant differences (**p < 0.01).

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