Fig. 4: Inhibited angiogenesis and activated NLRP3 inflammasome was induced by p-MAP4 in vitro. | Cell Death Discovery

Fig. 4: Inhibited angiogenesis and activated NLRP3 inflammasome was induced by p-MAP4 in vitro.

From: Microtubule associated protein 4 (MAP4) phosphorylation reduces cardiac microvascular density through NLRP3-related pyroptosis

Fig. 4

A Infection efficiency of MAP4 (Glu) or MAP4 (Ala) adenoviruses determined by western blotting; n = 5. B–D The statistical analysis of HA-MAP4, MAP4, and p-MAP4. ***p < 0.001 versus the CMV-null group. ###p < 0.001 versus the Ad-MAP4 (Ala) group. N.S. represents no statistical difference; n = 5. E Representative images of tube formation assay; n = 3. F Capillary length analysis of tube formation assay. ***p < 0.001 versus the CMV-null group. ##p < 0.001 versus the Ad-MAP4 (Ala) group. N.S. represents no statistical difference; n = 3. G Representative immunoblotting bands of NLRP3, ASC, caspase-1 (p10), and pro-caspase-1 in HUVECs; n = 5. H–K The statistical analysis of NLRP3, ASC, and caspase-1. *p < 0.05 and ***p < 0.001 versus the CMV-null group. ##p < 0.01 and ###p < 0.001 versus the Ad-MAP4 (Ala) group. N.S. represents no statistical difference; n = 5. L Representative confocal images of NLRP3/ASC colocalization in HUVECs. Scale bar, 10 µm; n = 3. M Representative confocal images of caspase-1 p10 in HUVECs. Scale bar, 10 µm; n = 3. N Statistical analysis of Pearson R value of NLRP3/ASC colocalization in HUVECs. **p < 0.01 versus the CMV-null group. ##p < 0.01 versus the Ad-MAP4 (Ala) group. N.S. represents no statistical difference; n = 3. (O) Statistical analysis of mean gray value of caspase-1 p10 in HUVECs. ***p < 0.001 versus the CMV-null group. ##p < 0.01 versus the Ad-MAP4 (Ala) group. N.S. represents no statistical difference; n = 3.

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