Fig. 2: Phylogenetic tree and scatter diagram showing clonal evolution in CLM.

The left panel exhibited phylogenetic tree and the right panel exhibited scatter plot in each patient. On the phylogenetic tree, the trunk (blue) indicates mutation shared by both primary tumor and liver metastases, red branch indicates metastasis-private mutations and the green branch indicates primary-private mutations, and the number on trunk or branch indicate amount of corresponding mutations. S-C, subclonal-clonal clonal pattern, 0-C, none-clonal clonal pattern, the genes in green are driver genes and those in red are recurrently mutated genes. m metastasis, p primary. In the scatter diagram, X-axis indicates the mutational CCF value in primary tumor, Y-axis represents mutational CCF value in liver metastasis, spots with different colors indicate the clonal type from primary tumor to liver metastases: red, clonal_clonal; green, clonal_subclonal; blue, subclonal_subclonal; purple, subclonal_clonal. CLM colorectal liver metastasis, CCF cancer cell fraction.