Fig. 5: MiR-508-5p directly targets CRISP3. | Cell Death Discovery

Fig. 5: MiR-508-5p directly targets CRISP3.

From: LINC01342 silencing upregulates microRNA-508-5p to inhibit progression of lung cancer by reducing cysteine-rich secretory protein 3

Fig. 5

A Binding site between miR-508-5p and CRISP3 was predicted at Targetscan. B Targeting relationship between miR-508-5p and CRISP3 was confirmed using dual luciferase reporter gene assay. C mRNA expression of CRISP3 in LC tissues was determined using RT-qPCR. D Protein expression of CRISP3 in LC tissues was determined using Western blot analysis. E mRNA expression of CRISP3 in LC cells was determined using RT-qPCR. F Protein expression of CRISP3 in LC cells was determined using Western blot analysis. G Correlations between miR-508-5p and CRISP3, and between LINC01342 and CRISP3 were analyzed using Pearson test. H CRISP3 mRNA expression after miR-508-5p upregulation was detected using RT-qPCR. I CRISP3 protein expression after miR-508-5p upregulation was detected using Western blot analysis; *P < 0.05 vs. the mimic NC group; #P < 0.05 vs. 16HBE cells.

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