Fig. 6: Intrathymic injection of Jagged1 led to thymic atrophy. | Cell Death Discovery

Fig. 6: Intrathymic injection of Jagged1 led to thymic atrophy.

From: Circulating mature dendritic cells homing to the thymus promote thymic epithelial cells involution via the Jagged1/Notch3 axis

Fig. 6

For A to F: Thymic atrophy after intrathymic injection of rmJagged1-hFc (n = 3). The same volume of PBS was used as a control (n = 3). A Thymus morphology and size. B Total numbers of thymocytes were calculated. C Thymocytes were stained with PE-conjugated anti-CD4 and APC-labeled anti-CD8 antibodies. The thymocyte subpopulations were analyzed by FCM. D The percentages and absolute numbers of the indicated thymocyte populations were calculated. E The cells isolated from the thymus of recipient mice were stained with Alexa Fluor 488-conjugated anti-CD45 and APC-labeled anti-EpCAM antibodies, respectively. CD45-EpCAM+ TECs were evaluated by FCM. F The percentages and absolute numbers of CD45-EpCAM+ cells were calculated. All data are from three independent experiments. Representative figures of these three experiments are shown. Data are represented as the mean ± SD. *P < 0.05, ***P < 0.001 compared with the control group.

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