Fig. 1: MSC-EVs characterization and tracking in APP / PS1 mice.

A Western blots of CD9, CD63, TSG101 and calnexin in MSC-EVs and MSCs (control). B, B1 transmission electron microscopy (TEM) images show the morphology of MSC-EVs (B, bar = 200 nm; B1, bar = 100 nm). C Nanoparticle tracking analysis (NTA) of size distribution for MSC-EVs. D Representative images show the location of C5 Maleimide-Alexa 594 (CM-A954) labeled MSC-EVs (red, white arrowheads) in the hippocampal neurons (green) in vivo (bar = 20 μm), the square area appears with higher magnification in the image on the right (bar = 5 μm).