Fig. 1: The circβ-catenin expression is upregulated in GBC tissues and cells. | Cell Death Discovery

Fig. 1: The circβ-catenin expression is upregulated in GBC tissues and cells.

From: Circβ-catenin promotes tumor growth and Warburg effect of gallbladder cancer by regulating STMN1 expression

Fig. 1

A The relative expression of circβ-catenin in gallbladder cancer tissues and adjacent normal tissues was detected by qRT-PCR analysis. B The correlation between the expression of circβ-catenin and over survival (OS) of GBC patients analyzed by the Kaplan–Meier method and log-rank test. C the relative expression of circβ-catenin in GBC cells (GBC-SD, SGC-996, NOZ, and OCUG-1) and H69 cell was determined by qRT-PCR analysis. D The expression of circβ-catenin was assessed by qRT-PCR in the nuclear and cytoplasm fractions in NOZ or SGC-996 cell lines. E RNase R was used to pretreat the RNAs and we found that the circular form of circβ-catenin was resistant to RNase R in four GBC cell lines compared with control group. F The subcellular localization of circβ-catenin detected by FISH assay. G RNA was isolated and RT-PCR with Sanger sequencing confirmed the circular form in NOZ cells and comes from exon regions. Data are shown as mean ± SD. *P < 0.05, **P < 0.01.

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