Fig. 5: circβ-catenin targets miR-223/STMN1 in GBC cells. | Cell Death Discovery

Fig. 5: circβ-catenin targets miR-223/STMN1 in GBC cells.

From: Circβ-catenin promotes tumor growth and Warburg effect of gallbladder cancer by regulating STMN1 expression

Fig. 5

A MiR-223 showed complementary base pairing with circβ-catenin using the online software tools circinteractome (http://circinteractome.nia.nih.gov). B Luciferase reporter assay illustrated the correlation within circβ-catenin-WT and miR-223. C 3′-terminal-biotinylated-miR-223 probe that was verified to pull down circβ-catenin in NOZ and SGC-996 cells. D, E The miR-223 expression was detected by qRT-PCR after transfection of NOZ and SGC-996 cells with shRNA-NC, shRNA-1, or shRNA-2, or after transfection of GBC-SD cells with pLCDH-vector or pLCDH-circβ-catenin. F The WT and corresponding MUT of the STMN1 mRNA 3′ UTR were constructed targeting miR-223. G, H Luciferase reporter assays were performed in NOZ and SGC-996 cells transfected with pLCDH-vector, pLCDH-circβ-catenin, miR-223 mimic, or co-transfected with miR-223 mimic + pLCDH-circβ-catenin and STMN1-WT or STMN1-MUT reporter vector. Data are shown as mean ± SD. *P < 0.05. **P < 0.01. n.s., not significant.

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