Fig. 8: Downregulation of NEAT1 and overexpression of miR-139-5p suppressed the expression of profibrotic markers in murine fibrosis models. | Cell Death Discovery

Fig. 8: Downregulation of NEAT1 and overexpression of miR-139-5p suppressed the expression of profibrotic markers in murine fibrosis models.

From: miR-139-5p sponged by LncRNA NEAT1 regulates liver fibrosis via targeting β-catenin/SOX9/TGF-β1 pathway

Fig. 8

A The liver tissues from fibrotic mice injected with Ad-Con or Ad-shNEAT1 were treated with immunohistochemical staining, n = 6 mice for each group, original magnification × 200. B The liver tissues from fibrotic mice injected with agomir control or ago-miR-139-5p were treated with immunohistochemical staining, n = 6 mice for each group, original magnification × 200. C The mRNA expression levels of α-SMA, Collagen-I and TIMP-1 were detected in the liver tissues from the fibrotic mice injected with Ad-Con or Ad-shNEAT1 by qRT-PCR. n = 6/group. D The mRNA expression levels of α-SMA, Collagen-I and TIMP-1 were detected in liver tissues from the fibrotic mice injected with agomir control or ago-miR-139-5p by qRT-PCR. n = 6/group. E The protein levels of β-catenin, SOX9, TGF-β1, α-SMA, Collagen-I, and TIMP-1 in liver tissues from untreated mice and fibrotic mice transfected with Ad-Con or Ad-shNEAT1 were detected by western blotting. Representative of three experiments. F The protein levels of β-catenin, SOX9, TGF-β1, α-SMA, Collagen-I, and TIMP-1 in liver tissues from untreated mice and fibrotic mice transfected with the agomir control and ago-miR-139-5p were detected by western blotting. Representative of three experiments. Graph represents mean ± SD. *P < 0.05, **P < 0.01.

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