Fig. 3: ATO and PARP inhibitors induce markers of DNA damage in SKOV3 and CAOV3 cells.
From: Arsenic compound sensitizes homologous recombination proficient ovarian cancer to PARP inhibitors
SKOV3 (A) and CAOV3 (B) cells were treated with DMSO, ATO, niraparib, olaparib, fluazolepali, combination of ATO and niraparib, combination of ATO and olaparib, or the combination of ATO and fluazolepali using the IC50 of each drug for 48 h. Co-IF for γH2AX was performed. Cells were counterstained with DAPI. Representative images are shown of three biologically independent experiments. Magnification is ×60. Scale bar is 10 μm. Quantization of γH2AX expression was analyzed. The γH2AX fold change was evaluated by number of foci/cell and normalized with DMSO-treated group. A total of 50 cells from each slide were counted. Error bars represent mean ± SD. **P < 0.01; ***P < 0.001.
