Fig. 4: Rdx binds Diap1.
From: The Hh pathway promotes cell apoptosis through Ci-Rdx-Diap1 axis
a Flag-tagged Rdx protein could pull down Myc-tagged Diap1 protein in 293 T cells. b 293 T cells transfected with Myc-Diap1 and Flag-Rdx were divided into two parts to carry out IP using mouse IgG or mouse anti-Flag antibody. The following WB results showed that only mouse anti-Flag antibody was able to pull down Flag-Rdx and Myc-Diap1. c The schematic drawing shows the domains in Rdx protein and the truncated fragments used in subsequent co-IP assays. d Flag-tagged Diap1 protein pulled down Rdx-MATH protein, but not Rdx-BTB protein in 293 T cells. e Myc-tagged Rdx-MATH interacted with Flag-Diap1. f Diap1 protein contained two SBCs. g, h Reciprocal co-IP experiments showed mutation of SBCs abolished Diap1 interaction with Rdx-MATH. Above all, arrowheads indicate heavy IgG, and WCL represents whole cell lysate.
