Fig. 5: Rdx fails to destabilize Diap1.
From: The Hh pathway promotes cell apoptosis through Ci-Rdx-Diap1 axis
a A wing disc expressing diap1 by MS1096 was stained with Diap1 antibody. b A wing disc simultaneous expressing diap1 and HA-rdx was stained to show Diap1 (green) and HA (red). Of note, Rdx did not decrease Diap1 protein. c A control wing disc from tub-Myc-diap1 was stained to show Ci (red) and Myc (green) antibodies. tubulin promoter drives Myc-diap1 wide expression in the wing disc. d A wing disc expressing rdx by Ap-gal4 under tub-Myc-diap1 background was stained with Ci (red) and Myc (green) antibodies. Overexpression of rdx did not affect Myc-tagged Diap1 protein. e IB assays of lysates from control wing disks or wing disks expressing HA-rdx with nub-gal4 driver. Approximately 50 wing disks were dissected, lysed, and blotted with indicated antibodies. Actin acts as a loading control. Relative densities of Myc-Diap1 bands were shown below. Data were presented as means ± SD from four independent experiments.
