Fig. 6: Rdx promotes K63-linked ubiquitination on Diap1 protein.
From: The Hh pathway promotes cell apoptosis through Ci-Rdx-Diap1 axis
a Rdx enhanced ubiquitination of Diap1. b Rdx failed to promote Diap1 ubiquitination when HA-Ub-K63R was expressed in 293T cells. Notably, K63 in Ub was important for Rdx-induced Diap1 ubiquitination. c Rdx promoted K63-linked polyubiquitination on Diap1. d Rdx elevated K63-linked ubiquitination of Diap1 protein, while Rdx-Δ3box failed to do so. e Overexpression of ub-K63R or ub-K0 enlarged GMR-hid eye, while ub-K48R did not. ci-RNAi acts as a positive control. Quantification analyses of eye sizes (n = 25) were shown below. Scale bars: 200 μm for all eyes. Above all, arrowheads indicate heavy IgG, and WCL represents whole cell lysate.
