Fig. 3: PP down-regulated MGMT expression and enhanced the TMZ chemotherapeutic effect on GBM cell lines. | Cell Death Discovery

Fig. 3: PP down-regulated MGMT expression and enhanced the TMZ chemotherapeutic effect on GBM cell lines.

From: Pyrvinium pamoate regulates MGMT expression through suppressing the Wnt/β-catenin signaling pathway to enhance the glioblastoma sensitivity to temozolomide

Fig. 3

A The PP role in MGMT protein level was measured through western blotting. B The PP role in MGMT mRNA level was measured through quantitative RT-PCR (n = 3). C LN18 and T98G cell viability under PP and TMZ treatment at different contents (n = 3). CalcuSyn 2.0 was adopted to generate the curves. The Fa-CI plots showed the value of combination index (CI) in every fractional effect, and PP was synergized with TMZ (CI < 1). D Surface plots of LN18 and T98G cells treated with TMZ, PP, or combined TMZ + PP shown in 2D and 3D. Plots were generated using Combenefit program by applying HSA model. E MGMT protein levels in over-expressing MGMT and control cells were examined within LN18 and T98G cells (top panel). Densitometric analysis of MGMT/β-actin expression fold change (bottom panel) (n = 3). F Effect of MGMT overexpression on cell viability under TMZ, PP, or TMZ combined PP treatment (n = 3). Results were presented in the manner of mean ± SD. *p < 0.05; **p < 0.01; ***p < 0.005; ****p < 0.001.

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