Fig. 5: PP-induced MGMT suppression and weakened-resistance to TMZ of GBM cells mediated by β-catenin. | Cell Death Discovery

Fig. 5: PP-induced MGMT suppression and weakened-resistance to TMZ of GBM cells mediated by β-catenin.

From: Pyrvinium pamoate regulates MGMT expression through suppressing the Wnt/β-catenin signaling pathway to enhance the glioblastoma sensitivity to temozolomide

Fig. 5

A MGMT and β-catenin protein levels in β-catenin knockdown and control cells were examined within LN18 and T98G cells (left panel). Densitometric analysis of β-catenin and MGMT expression (right panel) (n = 3). B Cell viability in β-catenin knockdown and control cells under TMZ treatment within LN18 and T98G cells (n = 3). C Effect of MGMT overexpression on cell viability in β-catenin knockdown and TMZ treatment groups within LN18 and T98G cells (n = 3). D MGMT and β-catenin protein levels in β-catenin overexpression and control cells under PP treatment. E Densitometric analysis of β-catenin/β-actin and MGMT/β-actin expression relative fold change in LN18 (top panel) and T98G cells (bottom panel) (n = 3). F Effect of β-catenin overexpression on cell viability under PP or PP combined TMZ treatment within LN18 and T98G cells (n = 3). G Schematic diagram for the MGMT promoter luciferase reporter plasmids (P-755/−39 ML, P-1346/−39 ML, and P-1989/−39 ML) possessed desired candidate Tcf/Lef-binding site numbers. H MGMT promoter luciferase reporter activity was measured following PP treatment at different contents in LN18 cells (n = 3). Data were presented in the manner of mean ± SD. *p < 0.05; **p < 0.01; ***p < 0.005.

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