Fig. 7: In order to verify whether LHPP could inhibit migration and invasion abilities of colorectal cancer cells via inactivating phosphorylation of Smad3 specifically or not. | Cell Death Discovery

Fig. 7: In order to verify whether LHPP could inhibit migration and invasion abilities of colorectal cancer cells via inactivating phosphorylation of Smad3 specifically or not.

From: LHPP suppresses colorectal cancer cell migration and invasion in vitro and in vivo by inhibiting Smad3 phosphorylation in the TGF-β pathway

Fig. 7

a, b The Transwell assay was used for evaluating the influence of SIS3 (details described before) on TGF-β1-mediated cell migration and invasion abilities (matrix gel 80 μl, dilution 1:8, 8-μm pore, scale bar, 100 μm, ×100). c, d Quantification results were determined using the Graphpad Prism 6 software. Migrated and invasive cells were stained and counted in at least five microscope fields. e, f Expression levels of EMT-related proteins in colorectal cancer cell lines. A significant difference could be observed not only in the overexpression group but also in the downexpression group with or without TGF-β1 treatment. g Potential mechanism of LHPP in migration and invasion abilities of the colorectal cancer cell. h–k Relative expression levels of biomarkers were calculated using Image J and Graphpad Prism 6 software. *P < 0.05, **P < 0.01, ***P < 0.001, ****P < 0.0001.

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