Fig. 2: miR-539 interacted with TIPE. | Cell Death Discovery

Fig. 2: miR-539 interacted with TIPE.

From: miR-539 activates the SAPK/JNK signaling pathway to promote ferropotosis in colorectal cancer by directly targeting TIPE

Fig. 2

a Putative wild-type (Wt) miR-539 binding sites in the 3ʹ-UTR of TIPE. b Relative luciferase activities were analyzed in 293 T cells cotransfected with Wt or Mut reporter plasmids and miR-539 mimic or miR-NC and miR-539 inhibitor or inhibitor-NC. c Expression level of TIPE in HCT116 cell lines after the transfection with miRNA-539 mimic or miR-NC and SW480 cells transfected with miR-539 inhibitor or inhibitor-NC was analyzed by RT-qPCR. d Expression level of TIPE in HCT116 cell lines after the transfection with miRNA-539 mimic or miR-NC and SW480 cells transfected with miR-539 inhibitor or inhibitor-NC was analyzed by western blot. e miR-539 and TIPE expression in 26 pairs of CRC tissues and the corresponding adjacent normal tissues were analyzed by RT-qPCR. Data are presented as the mean ± SD. *p < 0.05; **p < 0.01; ***p < 0.001; ****p < 0.0001; ns no significant difference.

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