Fig. 4: miR-539 regulates the expression of GPX4 through TIPE. | Cell Death Discovery

Fig. 4: miR-539 regulates the expression of GPX4 through TIPE.

From: miR-539 activates the SAPK/JNK signaling pathway to promote ferropotosis in colorectal cancer by directly targeting TIPE

Fig. 4

a GPX4 expression in 32 normal tissues and 33 tumor tissues was analyzed based on GEO (GSE146009). **p < 0.01. b GPX4 expression in 26 pairs of CRC tissues and the corresponding adjacent normal tissues was analyzed by qRT-qPCR. **p < 0.01. c, d Expression level of GPX4 in HCT116 cell lines after the transfection with miRNA-539 mimic or miR-NC and SW480 cells transfected with miR-539 inhibitor or inhibitor-NC was analyzed by qRT-qPCR and western blot. e Expression level of GPX4 in shCtrl and shTIPE HCT116 cells was analyzed by RT-qPCR and western blot. f Representative images of TIPE and GPX4 expression in CRC tumor tissues and normal tissues were analyzed by IHC and the percentage contribution of positive was calculated. Data are presented as the mean ± SD. *p < 0.05; **p < 0.01; ***p < 0.001; ****p < 0.0001.

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