Fig. 5: miR-539 regulates GPX4 through promoting the activation of SAPK/JNK by targeting TIPE. | Cell Death Discovery

Fig. 5: miR-539 regulates GPX4 through promoting the activation of SAPK/JNK by targeting TIPE.

From: miR-539 activates the SAPK/JNK signaling pathway to promote ferropotosis in colorectal cancer by directly targeting TIPE

Fig. 5

a HCT116 and SW480 cells were transfected with miR-539 mimic or miR-NC and miR-539 inhibitor or inhibitor-NC. After transfection for 24 h, the expression levels of TIPE, GPX4, p-SAPK/JNK and p53 were measured with western blot analysis. b HCT116 and SW480 cells were transfected with miR-539 mimic or miR-NC and miR-539 inhibitor or inhibitor-NC for 24 h, treat miR-NC and miR-539 inhibitor cells with inhibitor of JNK SP600125 (20 μM), the expression levels of TIPE, GPX4, p-SAPK/JNK and p53 were measured with western blot analysis. c The expression levels of TIPE, GPX4, p-SAPK/JNK and p53 were measured with western blot analysis in shCtrl, shTIPE and shTIPE treated with SP600125 (20 μM) for 24 h HCT116 cells. The value displayed above the WB result is the OD value after quantitative analysis. d Schematic diagram representing that downregulated miR-539 regulates GPX4 through inhibiting the activation of SAPK/JNK in CRC by targeting TIPE, afterwards, to inhibit ferroptosis and promote proliferation.

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