Fig. 2: Immunofluorescence co-localization of TH with p-α-syn in midbrain, as well as p-α-syn with NF and GFAP in sciatic nerves of MPTP-induced mice. | Cell Death Discovery

Fig. 2: Immunofluorescence co-localization of TH with p-α-syn in midbrain, as well as p-α-syn with NF and GFAP in sciatic nerves of MPTP-induced mice.

From: Phosphorylated α-synuclein aggregated in Schwann cells exacerbates peripheral neuroinflammation and nerve dysfunction in Parkinson’s disease through TLR2/NF-κB pathway

Fig. 2

A A remarkable decline of TH+ neurons co-localized with p-α-syn was noted in SNpc of MPTP group compared to saline group; p-α-syn was located in the cytosol, the soma of TH+ neurons or fibers of non-TH+ neurons. Scale bar, 1 µm. B The p-α-syn in sciatic nerves of MPTP group was significantly increased than saline group. NF was used to label axons, showing dotted or granular; p-α-syn was expressed as puncta, ring or liner shape, partly in axons. Scale bar, 2 µm. C GFAP was a marker of SCs in myelin sheath showing ring or half ring shape around axons; p-α-syn was mainly distributed in SCs. Scale bar, 2 µm. TH: tyrosine hydroxylase; p-α-syn: phosphorylated α-synuclein; NF: neurofilament 200 Kd; GFAP: glial fibrillary acidic protein; MPTP, 1-methyl-4-phenyl-1,2,3,6-tetrahydropyridine; SCs: Schwann cells.

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