Fig. 6: Exo-transmitted circCOG2 promotes CRC progression in vitro. | Cell Death Discovery

Fig. 6: Exo-transmitted circCOG2 promotes CRC progression in vitro.

From: Exosome-transmitted circCOG2 promotes colorectal cancer progression via miR-1305/TGF-β2/SMAD3 pathway

Fig. 6

A The illustration of establishing CRC cell lines with high metastatic potential or low metastatic potential. B, C The metastatic potential of CRC cells with different metastatic potential was confirmed by transwell assay. D Western blot showed the marker of exosomes. E NTA showed the size of exosomes. F TEM showed the microstructure of exosomes (white arrows). G The exosomes were stained by PKH67 (green fluorescence) were cocultured with CRC cells, and cell nuclear were stained by DAPI (blue fluorescence). H The expression of circCOG2 in cell cocultured with PBS, HM-EXO or HM-si-circCOG2-EXO. I–L CCK-8 and colony formation assays indicated exo-transmitted circCOG2 promoted the proliferation of CRC cells with low metastatic potential. M, N Transwell assay indicated that exo-transmitted circCOG2 promoted migration and invasion of CRC cells with low metastatic potential. O Exosome-transmitted circCOG2 promoted EMT and TGF-β2/SMAD3 pathway. *P < 0.05; **P < 0.01; ***P < 0.001; ****P < 0.0001; P-values were calculated by Student’s t-test.

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