Fig. 5: The RCN1 EFh1/2 domain binds with GRP78 and promotes the dissociation of GRP78 from IRE1. | Cell Death Discovery

Fig. 5: The RCN1 EFh1/2 domain binds with GRP78 and promotes the dissociation of GRP78 from IRE1.

From: RCN1 induces sorafenib resistance and malignancy in hepatocellular carcinoma by activating c-MYC signaling via the IRE1α–XBP1s pathway

Fig. 5

A Immunoblot analysis of the interaction between GRP78 and IRE1α using immunoprecipitates from sorafenib-resistant Huh7 cells with or without RCN1 silencing. B Immunoblot analysis of complex formation between endogenous RCN1 and GRP78 immunoprecipitated from sorafenib-resistant Huh7 cells. C Schematic representation of the whole-length and deletion RCN1 mutants. D Immunoblot analysis of complex formation between endogenous GRP78 and HA-tagged mutant RCN1. E Immunoblot analysis of IRE1α phosphorylation and XBP1s protein levels in sorafenib-resistant Huh7 cells transfected with whole-length and deletion RCN1 mutants. Data are presented as the means ± SEM of three independent experiments. ns not significantly different. **P < 0.01; ***P < 0.001; ****P < 0.0001, t-test.

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