Fig. 8: CircETFA regulates CCL5 mRNA by recruiting EIF4A3. | Cell Death Discovery

Fig. 8: CircETFA regulates CCL5 mRNA by recruiting EIF4A3.

From: CircETFA upregulates CCL5 by sponging miR-612 and recruiting EIF4A3 to promote hepatocellular carcinoma

Fig. 8

A The activity of the luciferase reporter gene of wild-type or mutant CCL5 3’-UTR was tested in dual-luciferase reporter experiments. B The protein levels of CCL5, Akt, p-Akt, FoxO6, CyclinD1, and MMP7 were measured after transfecting HCC cells with EIF4A3 vector and si-EIF4A3. C The half-life of CCL5 was detected after transfecting HCC cells with EIF4A3 vector and si-EIF4A3. D RIP was conducted to determine whether circETFA can recruit EIF4A3 to CCL5 mRNA. E, F The half-life of CCL5 was detected after co-transfecting HCC cells with si-circETFA/EIF4A3 vector or circETFA/si-EIF4A3. G CircETFA increases the half-life of CCL5 by recruiting EIF4A3 and acting as a sponge of hsa-miR-612. CircETFA can upregulate CCL5 in HCC cells, thereby regulating key downstream effectors such as the PI3K/Akt pathway and FoxO6 to promote HCC cell proliferation, invasion, and migration. The data are shown as mean ± SD. *P < 0.05, **P < 0.01, ***P < 0.001.

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