Fig. 4: HMGA1 positively regulates RAD51 expression in CCA cells.
From: Overexpression of HMGA1 confers radioresistance by transactivating RAD51 in cholangiocarcinoma
A Gene Ontology (GO) analysis of differentially expressed genes (2473 up- and 2058 downregulated) in RNA-seq data of HUCCT1 cells with HMGA1 knockdown. B, C Forty-five and 21 differentially expressed DNA repair genes were filtered from RNA-seq data and TCGA data, respectively. D The homologous recombination pathway was enriched in both RNA-seq and TCGA data. E Correlation analysis of HMGA1 and RAD51 expression in TCGA-CCA samples (R = 0.39, P = 0.0089). F Validation of the expression of RAD51 in HUCCT1 and QBC939 cell lines with HMGA1 overexpression and knockdown by qPCR. G The protein levels of RAD51 and HMGA1 were measured by western blot in HUCCT1 and QBC939 cells with HMGA1 overexpression or knockdown. The P-value was obtained by Student’s t test. The results represent the mean ± S.D. of three independent experiments. *P < 0.05, **P < 0.01, ***P < 0.001.
