Fig. 5: HMGA1 directly binds to the RAD51 promoter and induces its transcription.
From: Overexpression of HMGA1 confers radioresistance by transactivating RAD51 in cholangiocarcinoma
A qPCR analysis of ChIP samples in HUCCT1 cells with or without HMGA1 knockdown using the HMGA1 antibody or IgG. Fragment 1 (F1) and Fragment 3 (F3) were predicted targets of HMGA1, and Fragment 2 (F2) was the negative control. The P-value was obtained by Student’s t test. The results represent the mean ± S.D. of three independent experiments. **p < 0.01, ***p < 0.001. B Luciferase activity was measured in HEK293T cells transfected with HMGA1 knockdown or control plasmids in combination with pGL4 plasmids containing the full long (FL) or truncated (E1 and E2) promoter region (n = 5 biologically independent samples). C Luciferase activity was measured in HEK293T cells transfected with HMGA1 knockdown or control plasmids in combination with pGL4 plasmids containing the mutation of potential HMGA1 binding sites promoter region (−1756/−1739 M1; −964/−939 M2; double mutant: M3). Data are presented as means ± S.D. *p < 0.05, **p < 0.01, ***p < 0.001, and ****p < 0.0001.
