Fig. 7: NPs induce dopaminergic differentiation in a hiPSCs-derived neuronal population obtained from DONOR B. | Cell Death Discovery

Fig. 7: NPs induce dopaminergic differentiation in a hiPSCs-derived neuronal population obtained from DONOR B.

From: Natriuretic peptides are neuroprotective on in vitro models of PD and promote dopaminergic differentiation of hiPSCs-derived neurons via the Wnt/β-catenin signaling

Fig. 7

A, B Confocal microscopy images showing the effect of 24 h treatments with NPs, performed at day 31 (Step 1; A) or at day 38 (Step 2; B) of differentiation, on the expression and intracellular distribution of TH (green hue) and β-catenin (red hue), compared with the untreated control. Merged images of β-catenin/TH double immunofluorescent staining and nuclei counterstaining with Hoechst (blue hue) are also shown. Bars 25 µm. C, D WB analyses of the effects of NPs treatments, at day 31 (Step 1; C) or at day 38 (Step 2; D) of total β-catenin, total and phosphorylated TH (pTH at Ser40) and of unglycosylated (50 kDa), mono- (58 kDa), di- (62 kDa), and tri- (75 kDa) glycosylated forms of the dopamine transporter DAT. In the densitometric analysis, values were normalized to β-actin, or vs. the total levels of total TH for phosphoTHS40. Results are the mean ± SD from three independent experiments (n = 3). Significance vs. the untreated control (one-way ANOVA + Tukey multiple comparison test): *p < 0.05, **p < 0.01, ***p < 0.001. Additional results from the one‐way ANOVA test used to analyze the differences between groups are reported in Supplementary Data.

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