Fig. 2: PSMC2 shRNAs transfections.
From: PSMC2 knockdown suppressed tumor progression of skin cutaneous melanoma
A The PSMC2 expression profile at the protein level (upper part) and mRNA level (bottom part) in human melanoma cell lines (A375, SK-MEL-1, SK-MEL-28) and human epidermal melanocytes (PIG1). B Western blot (upper part) and RT-qPCR (bottom part) were used to assess the transfection efficiency of PSMC2 shRNA-1 and shRNA-2 and shRNA-3 in A375 cells. C PSMC2 knockdown is associated with reduced proliferation in A375. CCK-8 assay was performed to measure the rate of cell proliferation of A375 after transfected with shPSMC2-1, shPSMC2-2, and shPSMC2-3 for 5 days. D, E RT-qPCR and Western blot were used to detect the expression of PSMC2 in A375 cells after shPSMC2 transfection. F, G RT-qPCR and Western blot were used to detect the expression of PSMC2 in SK-MEL-28 cells after shPSMC2 transfection. Each independent experiment was repeated at least three times. Data were expressed as the mean ± SD. *p < 0.05, **p < 0.01 and ***p < 0.001.
