Fig. 4: Knockdown of PSMC2 inhibits cell migration and DNA damage in SKCM cells and tumor growth in vivo.
From: PSMC2 knockdown suppressed tumor progression of skin cutaneous melanoma
A, B Scratch test assays were performed to detect cell migrative capacities of A375 (at 0 and 24 h) and SK-MEL-28 cells (at 0 and 8 h) after shPSMC2 transfection. C, D Transwell assays were performed to detect cell migrative capacities of A375 and SK-MEL-28 cells after shPSMC2 transfection. E Western blotting was used to evaluate the protein expressions of ATM, γ-H2AX, and CDKN1A in PSMC2 silenced A375 and SK-MEL-28 cells. GAPDH was used as a loading control. Histogram representing indicated the results of three independent experiments. F Tumor volume growth curves for subcutaneous xenografts and data were obtained from 10 animals in each group. G After scarification, xenograft excised tumors were recorded and the bar graph presented the excised tumor weights derived from control or PSMC2 silenced A375 cells. n = 10. Each independent experiment was repeated at least three times. Data were expressed as the mean ± SD. The statistical significance of the data between two groups was compared using Student’s t-test. *p < 0.05, **p < 0.01 and ***p < 0.001.
