Fig. 5: The effect of inhibiting proteasome and exogenously enforced PSMC2 expression on cell proliferation, cell cycle and apoptosis.
From: PSMC2 knockdown suppressed tumor progression of skin cutaneous melanoma
After treatment with MG132 or OE-PSMC2 plasmids, (A, B) The mRNA levels of PSMC2 were assessed by real-time PCR in A375 and SK-MEL-28 cells. C, D) The protein levels of PSMC2 were determined by western blot in A375 and SK-MEL-28 cells. E, F The consequent influences on SKCM cell proliferation of A375 and SK-MEL-28 cells were separately determined by the CCK-8 assay. G, H Flow cytometry analysis was used to evaluate the cell cycle progression and cell fractional quantification in the G0/G1, S, and G2/M phases were shown in the diagrams. I, J Apoptosis via quantifying Annexin FITC positive cells were shown and the apoptotic rate was calculated as the percentage of Annexin APC positive cells. Each independent experiment was repeated at least three times. Data were expressed as the mean ± SD. *p < 0.05, **p < 0.01 and ***p < 0.001.
