Fig. 4: Wip1 interacted with BST2. | Cell Death Discovery

Fig. 4: Wip1 interacted with BST2.

From: Wip1 regulates the immunomodulatory effects of murine mesenchymal stem cells in type 1 diabetes mellitus via targeting IFN-α/BST2

Fig. 4

A The RNA transcripts heatmap of Wip1+/+MSCs and Wip1−/− MSCs was displayed by Microarray analysis. B The upregulated genes in Wip1+/+MSCs and Wip1−/−MSCs were determined by qPCR. C–F Western blot was used to confirm the protein expression of Wip1 and BST2 in Wip1+/+MSCs and Wip1−/− MSCs. G Wip1-EGFP and EGFP plasmids were transfected into 293 T cells, and the positive cells were observed by fluorescence microscope. H The transfection efficiency of Wip1-EGFP and EGFP plasmids in 293 T cells was detected by flow cytometry. I Western blot assay was utilized to determine the endogenous interaction between Wip1 and BST2 in 293 T cells. J Co-IP assay was used to detect the interaction between endogenous Wip1 and BST2 in MSCs cells. *P < 0.05.

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