Fig. 2: MMP1 contributed to the proliferation of colon cancer cells by accelerating the cell cycle transition from G0/G1 to S and G2/M phase.
From: Tumor-associated macrophages (TAMs) depend on MMP1 for their cancer-promoting role
A Comparison of the mRNA levels of MMP1/2/9 between U937 cells and TAMs by RT-PCR. B Comparison of the protein levels of MMP1/2/9 between U937 cells and TAMs by western blotting analysis. C The release of MMP1/2/9 in U937 cells and TAMs detected by ELISA assay. D Western blotting analysis for MMP1 in MMP1-overexpressing U937 cells or MMP1-knockdown TAMs. E The release of MMP1 in MMP1-overexpressing U937 cells or MMP1-knockdown TAMs detected by ELISA assay. F The effect of co-culture with TAM-shMMP1 or U937-MMP1 on the growth and viability of HT-29 cells. G HT-29 and Caco-2 cells were treated with 0, 25, 50, and 75% TAMs- shMMP1-CM for different time. The effect of treatment with TAMs-shMMP1-CM on the viability of HT-29 and Caco-2 cells was detected by cell viability assays. H, I The effect of co-culture with TAMs-shMMP1 (H) or treatment with TAMs- shMMP1-CM (I) on the cell cycle distribution of HT-29 and Caco-2 cells detected by FACS analysis. All data are presented as the mean ± SD from three independent experiments. *P < 0.05.
