Fig. 2: Inhibiting/promoting β1-AR endocytosis enhanced/weakened the sustained activation of β1-AR. | Cell Death Discovery

Fig. 2: Inhibiting/promoting β1-AR endocytosis enhanced/weakened the sustained activation of β1-AR.

From: Biased activation of β2-AR/Gi/GRK2 signal pathway attenuated β1-AR sustained activation induced by β1-adrenergic receptor autoantibody

Fig. 2

A The effect of the receptor endocytosis inhibitor Dynasore on the NE-induced increase in NRCMs beating frequency. n = 5/group, *P vs NE + Phe, P < 0.05. B An radioimmunoassay kit was used to detect the cAMP concentration in NRCMs after treatment with different stimuli. n = 4/group. *P vs NE + Phe, †β1-AA vs negative IgG, P < 0.05. C–F After overexpressing β-arrestin1 (β-arr1 OE) or β-arrestin2 (β-arr2 OE), the effect of β1-AA on β1-AR-GFP endocytosis was measured via TIRF. HL-1 cells were transiently transfected with β1-AR-GFP, combined with or without transfection with β-arrestin1-RFP (C and D) or β-arrestin2-RFP (E and F). C and E are typical pictures, and D and F are the corresponding statistical charts. Scale bar = 10 μm. n = 3–4/group. ‡P vs β1-AA, P < 0.05. Data in A–F were analyzed by two-way ANOVA with a Bonferroni post hoc test. G and H The impacts of β1-AA on intracellular Ca2+ in HL-1 cells with or without β-arr1 OE or β-arr2 OE. Fluo-4 (10 μM) was used as a Ca2+ indicator. n = 3–4/group.

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