Fig. 4: By activating β2-AR/Gi, ICI118551 promoted β1-AA-induced β1-AR endocytosis and prevented continuous β1-AR activation. | Cell Death Discovery

Fig. 4: By activating β2-AR/Gi, ICI118551 promoted β1-AA-induced β1-AR endocytosis and prevented continuous β1-AR activation.

From: Biased activation of β2-AR/Gi/GRK2 signal pathway attenuated β1-AR sustained activation induced by β1-adrenergic receptor autoantibody

Fig. 4

A, B The impact of ICI118551 on β1-AA-induced increased beating frequency and cAMP level of NRCMs, respectively. ICI118551 was added 10 min after administration of β1-AA. n = 3–6/group. *P vs β1-AA + ICI118551, P < 0.05 (two-way ANOVA with Bonferroni test). C The reversal effect of ICI118551 on the persistent rise in intracellular Ca2+ caused by β1-AA in HL-1 cells. HL-1 cells were pretreated with Fluo-4 for 90 min, ICI118551 was added, and then β1-AA was added after 5 min. n = 3/group. D and E The reversal effect of ICI118551 on the limited endocytosis of β1-AR caused by β1-AA. Scale bar = 10 μm. n = 3–6/group. †P vs β1-AA, P < 0.05 (two-way ANOVA with Bonferroni test). F illustrates that β2-AR/Gi-biased activation by ICI118551 could reverse the limited endocytosis and sustained activation of β1-AR induced by β1-AA.

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