Fig. 6: Activation of GRK2 enhanced the recruitment of β1-AR and β-arrestin stimulated by β1-AA. | Cell Death Discovery

Fig. 6: Activation of GRK2 enhanced the recruitment of β1-AR and β-arrestin stimulated by β1-AA.

From: Biased activation of β2-AR/Gi/GRK2 signal pathway attenuated β1-AR sustained activation induced by β1-adrenergic receptor autoantibody

Fig. 6

A The phosphorylation of GRK2 was detected after HL-1 cells were stimulated with ICI118551 alone or in combination with β1-AA for 5 min. n = 4/group. *P vs β1-AA, P < 0.05 (one-way ANOVA with Dunnett test). B The detection of PKA phosphorylation. n = 4/group. C, D TIRF was used to detect the recruitment of β1-AR and β-arrestin1/2 (above pictures), and Pearson’s coefficient (below tables) was calculated to reflect the colocalization between the two proteins. White arrows indicate the colocalization of β1-AR with β-arrestin1 (C) or β-arrestin2 (D). n = 3/group.

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