Fig. 2: CPNE1 is degraded through both the ubiquitin-proteasome pathway and the autophagy-lysosome pathway. | Cell Death Discovery

Fig. 2: CPNE1 is degraded through both the ubiquitin-proteasome pathway and the autophagy-lysosome pathway.

From: The regulation of CPNE1 ubiquitination by the NEDD4L is involved in the pathogenesis of non-small cell lung cancer

Fig. 2

A CPNE1 was highly expressed in lung cancer cell lines. B, C CPNE1 was rapidly degraded in HEK293T cells. HEK293T cells were transfected with the Flag-CPNE1 plasmid. After 48 h, cells were treated with 100 µg/ml cycloheximide/vehicle for the indicated times. Cell lysates were prepared for Western blot analysis. The relative density of the CPNE1 band was measured with ImageJ software. D The degradation of CPNE1 mediated by CHX was rescued by MG132. The indicated cell lines were seeded into six-well plates. After 24 h, cells were treated with 100 µg/ml CHX or 100 µg/ml CHX plus 10 µM MG132 for 6 h. Cell lysates were prepared for Western blot analysis. E, F Both MG132 and 3-MA can stabilize CPNE1. The indicated cell lines were seeded into six-well plates. After 24 h, cells were treated with 10 µM MG132 for 6 h and 1 mM 3-MA for 18 h. Cell lysates were prepared for Western blot analysis.

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