Fig. 5: NEDD4L interacts with the CPNE1 protein. | Cell Death Discovery

Fig. 5: NEDD4L interacts with the CPNE1 protein.

From: The regulation of CPNE1 ubiquitination by the NEDD4L is involved in the pathogenesis of non-small cell lung cancer

Fig. 5

A The co-immunoprecipitation assay proved the association between endogenous CPNE1 and NEDD4L. Co-immunoprecipitation was performed as indicated. B CPNE1 protein levels in si-NEDD4L NSCLC cells. C Immunofluorescence was used to detect the intracellular localization of CPNE1 and NEDD4L. The intracellular localization of CPNE1 (green) and NEDD4L(red) is shown. D Upregulation of CPNE induced by NEDD4L interference was reduced by MG132 treatment. A549 cells were transfected with si-NEDD4L or si-Control. MG132 was added 6 h prior to protein extraction. E NEDD4L protein levels in NEDD4L overexpression A549 cells. F CCK-8 assay of cell viability in A549 (OE-NEDD4L compared with OE-NC). G the results of clonogenic analysis of cell proliferation in A549 cells (OE-NEDD4L compared with OE-NC). H Representative images of the transwell assay results for cell migration and invasion in A549 cells (OE-NEDD4L compared with OE-NC). I NEDD4L decreases CPNE1 half-life in HEK 293 T cells. Cells were transfected with sg-Control and sg-NEDD4L. 100 μg/ml cycloheximide was added for indicated times prior to protein extraction. Image J was used to quantify the CPNE1 protein level. **P < 0.01; ***P < 0.001.

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