Fig. 1: CD5L deficiency alleviates APAP-induced liver injury in mice. | Cell Death Discovery

Fig. 1: CD5L deficiency alleviates APAP-induced liver injury in mice.

From: CD5L deficiency attenuate acetaminophen-induced liver damage in mice via regulation of JNK and ERK signaling pathway

Fig. 1

APAP was intraperitoneally injected into wild-type (WT) mice (n = 5 for each time point) at 300 mg/kg, and the CD5L level in the liver at specified time point was detected by A q-PCR and B western blotting; C Immunohistochemical staining of CD5L in liver sections of WT (n = 4 for each group) treated with PBS or APAP for 24 h, representative images are shown; D The expression of CD5L in human liver tissue (www.proteinatlas.org); E The diagram of CRISP/Cas9 CD5L knockout strategy; F The expression of CD5L protein in the liver of WT and CD5L-/- mice was detected by western blotting; G Immunohistochemical staining of CD5L in liver sections of WT or CD5L−/− mice (n = 4 for each group), representative images are shown; H, I Serum ALT and liver H&E staining at 8 h and 24 h after PBS or APAP injection in mice (n = 10 for each group). Necrotic area was measured by Image J. Representative images are shown. Scale: 100 μm. The results are presented as means ± SD of at least three independent experiments. *P < 0.05; **P < 0.01; ***P < 0.001.

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